Increased yield of PCR product from degenerate primers with nondegenerate, nonhomologous 5′ tails

Selecting Degenerate Multiplex PCR Primers

Single Nucleotide Polymorphism (SNP) Genotyping is an important molecular genetics technique in the early stages of producing results that will be useful in the medical field. One of the proposed methods for performing SNP Genotyping requires amplifying regions of DNA surrounding a large number of SNP loci. In order to automate a portion of this method and make the use of SNP Genotyping more wi...

5'-degenerate 3'-dideoxy-terminated competitors of PCR primers increase specificity of amplification.

Amplification of a product in PCR with specific primers may be viewed as an artificial Darwinian-type "selection of the fittest". In other selective systems, such as general evolution, immune system and probably brain cortex, the stringency of selection is not absolute but rather degenerate, with selection of many highly fit units, not limited, however, to only the fittest. In PCR also, anneali...

PCR with arbitrary primers

Amplification of Hsp90 homologs from plant-parasitic nematodes using degenerate primers and ramped annealing PCR.

Procedures for using PCR to amplify novel members of gene families from genomic DNA frequently involve the use of degenerate primers. Of key importance is the ability to produce ample quantities of specific PCR product while minimizing or eliminating nonspecific side reactions. Primer design protocols and software are improving, but finding the optimal amplification conditions often involves a ...

Degenerate Primers Facilitate the Detection and Identification of Potyviruses From the Northwest Region of Iran

Background: Potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. Potyviruses can be transmitted through plant sap, seeds and many aphid species. Due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. Objectives: This study compared the efficiencies of two couples of primers in the...